Poster 1: Work within this poster examined the relationship between arsenic contaminated skin cells and estradiol as a possible therapeutic. Using an in vitro bench top wound healing model known as the scratch assay, human skin cells were wounded and treated with arsenic ± estradiol. The results demonstrated a decrease in cellular migration rates for cells that were not treated with estradiol, while treated cells showed an increase in migration rates.
Link to Poster 1: https://drive.google.com/open?id=14wrUVbQoWeCeHojlv0WTe8etsKYahq2z
Link to Poster 1: https://drive.google.com/open?id=14wrUVbQoWeCeHojlv0WTe8etsKYahq2z
Poster 2: This poster further evaluated the impact of arsenic contamination on cellular migration by using the scratch assay and various environmentally relevant doses of arsenic. In addition to the scratch assay, human skin cells were contaminated with arsenic and later counted in order to determine the doubling rate for contaminated cells compared to non-contaminated control. The results demonstrated a decrease in cellular migration of cells exposed to the high end of our dosage, with the same being true for the doubling rate of our treated skin cells compared to controls.
Link to Poster 2: https://drive.google.com/open?id=1UtXA9goH_UOGutx8I3sW8DBU_9_KKKfe
Link to Poster 2: https://drive.google.com/open?id=1UtXA9goH_UOGutx8I3sW8DBU_9_KKKfe
Poster 3: The research in this poster is a continuation of the work that has taken place previously, but now critical cell functions and a decrease of arsenic concentrations in a chronic exposure environment were evaluated. Cell metabolism, proliferation, and migration all play a critical part in wound healing; the decrease in arsenic concentrations was used to be more relevant to the Environmental Protection Agency (EPA) maximum contaminant level (MCL) of arsenic in drinking water. The results demonstrated chronic exposure to environmentally relevant doses of arsenic decreased cellular migration, while a 24 exposure led to a decrease in metabolism and DNA content.
Link to Poster 3: https://drive.google.com/open?id=1LOcPSsdlzCKp3YaHWNXJD2tXtrP-8b9v
Link to Poster 3: https://drive.google.com/open?id=1LOcPSsdlzCKp3YaHWNXJD2tXtrP-8b9v
Publications
Over the years of working in the TERM lab on the arsenic wound healing project resulted in two publications that are both in press.
Publication 1: This an article that has been peer reviewed and published within the journal of Applied In Vitro Toxicology that analyzes how arsenic can decrease migration rates in cells, while estradiol has the potential as a possible therapeutic: www.ncbi.nlm.nih.gov/pmc/articles/PMC5881251/
Publication 1 Citation: Pinto, B. I., Lujan, O. R., Ramos, S. A., Propper, C. R., & Kellar, R. S. (2018). Estrogen Mitigates the Negative Effects of Arsenic Contamination in an In Vitro Wound Model. Applied in vitro toxicology, 4(1), 24–29. doi:10.1089/aivt.2017.0020
Publication 2: The second publication is closely related to the first, but here we focus on the scratch assay and how impactful it can be for in vitro wound healing studies. This publication is also unique considering it is based on video recordings that demonstrate all laboratory methods used for the scratch assay: https://www.jove.com/video/58838/in-vitro-scratch-assay-to-demonstrate-effects-arsenic-on-skin-cell
Publication 2 Citation: Pinto, B. I., Cruz, N. D., Lujan, O. R., Propper, C. R., Kellar, R. S. In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration. J. Vis. Exp. (144), e58838, doi:10.3791/58838 (2019).
Publication 1: This an article that has been peer reviewed and published within the journal of Applied In Vitro Toxicology that analyzes how arsenic can decrease migration rates in cells, while estradiol has the potential as a possible therapeutic: www.ncbi.nlm.nih.gov/pmc/articles/PMC5881251/
Publication 1 Citation: Pinto, B. I., Lujan, O. R., Ramos, S. A., Propper, C. R., & Kellar, R. S. (2018). Estrogen Mitigates the Negative Effects of Arsenic Contamination in an In Vitro Wound Model. Applied in vitro toxicology, 4(1), 24–29. doi:10.1089/aivt.2017.0020
Publication 2: The second publication is closely related to the first, but here we focus on the scratch assay and how impactful it can be for in vitro wound healing studies. This publication is also unique considering it is based on video recordings that demonstrate all laboratory methods used for the scratch assay: https://www.jove.com/video/58838/in-vitro-scratch-assay-to-demonstrate-effects-arsenic-on-skin-cell
Publication 2 Citation: Pinto, B. I., Cruz, N. D., Lujan, O. R., Propper, C. R., Kellar, R. S. In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration. J. Vis. Exp. (144), e58838, doi:10.3791/58838 (2019).